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Holmes's career, the rise and dissolution of her company, and the subsequent fallout are the subject of a book, Bad Blood: Secrets and Lies in a Silicon Valley Startup by the Wall Street Journal reporter John Carreyrou.After the end of her freshman year, Holmes worked in a laboratory at the Genome Institute of Singapore testing for severe acute respiratory syndrome through the collection of blood samples with syringes.She appeared on Jim Cramer's Mad Money the same evening the article was published.Cramer said, "The article was pretty brutal", to which Holmes responded, "This is what happens when you work to change things, first they think you're crazy, then they fight you, and then all of a sudden you change the world." In January 2016, the Centers for Medicare and Medicaid Services (CMS) sent a warning letter to Theranos after an inspection of its Newark, California laboratory uncovered irregularities with staff proficiency, procedures, and equipment. Department of Health and Human Services appeals board.It includes interviews and deposition tapes of key figures including Elizabeth Holmes; Ramesh "Sunny" Balwani; Christian Holmes (Elizabeth’s brother); Tyler Shultz (Theranos whistleblower); Theranos board members Bill Frist, Gary Roughead, Robert Kovacevich; and others.There is also an interview with Jeff Coopersmith, the attorney representing Balwani.The early credibility of Theranos was in part interpreted as an effect of Holmes's personal connections and ability to recruit the support of influential people including Henry Kissinger, Bill Clinton, George Shultz, James Mattis, and Betsy De Vos.

Several other expert medical professors told Holmes the same thing.

This was the first serious criticism of Theranos in the press, and a further series of articles by Carreyrou followed with more damning details.

Holmes denied all the claims, calling the newspaper a "tabloid" and promising the company would publish data on the accuracy of its tests.

On June 15, 2018, following an investigation by the U. Attorney's Office in San Francisco that lasted more than two years, a federal grand jury indicted Holmes and former Theranos chief operating officer and president Ramesh "Sunny" Balwani on nine counts of wire fraud and two counts of conspiracy to commit wire fraud. Prosecutors allege that Holmes and Balwani engaged in two criminal schemes, one to defraud investors, the other to defraud doctors and patients.

In June 2019, Bloomberg News reported Holmes and Balwani are looking into a possible defense strategy of blaming the media for the downfall of Theranos.

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